Featured Articles | A Bioscience Publishing Platform

Research Progress on Cloning and Mechanism of Rice Lesion Mimic Genes

The formation of rice lesion mimic was affected by gene mutation, metabolic disorder, accumulation of active oxygen, hormone imbalance, blocked ion channels, out of control programmed cell death process, abiotic stress and so on. The process of disease was usually accompanied by the improvemen... (28 Aug., 2020)

Cloning, Expression and Purification of MGG_14095 Gene of Magnaporthe grisea

Magnaporthe grisea is the most harmful pathogen in the world for rice and the main model organism for elucidating the molecular basis of plant fungal disease interaction. Carrying out relevant research of the growth and development of Magnaporthe grisea has a great significance for the con... (05 Jun., 2020)

Cloning and Prokaryotic Expression of LHY Gene from Soybean

LHY is the key gene of biological clock, we designed the corresponding primers which was based on soybean LHY candidate genes, we obtained the GmLHY2 gene by RT-PCR method to amplify the LHY genes in soybean cultivars. Its ORF span was 2250 bp, encoding was 749 amino acids. Then the G... (10 Apr., 2017)

Impact of Osmotic Stress on Biochemical and Physiological Parameters in Zea mays L. cv. Ganga Safed-2 Genotype

Treatment of maize leaf segments from etiolated seedlings with different concentrations of sorbitol during greening decreased the fresh weight, turgid weight and dry weight significantly, being indicated by higher R squared values obtained on correlation analysis. Concentration dependent decre... (13 Jul., 2016)

Molecular Characterization of Selected Maize (zea mays l.) Inbred Lines

Genetic knowledge of germplasm diversity among parental inbred lines has significant impact in the development of improved maize hybrids in the breeding program. The aim of our study was to assess maize inbred lines for variability in molecular traits and to estimate genetic distance among differ... (30 Oct., 2015)

Assessment of Variability and Identification of Transgressive Segregants for Yield and Yield Component Traits in Early Segregating Generations of Chickpea

In order to compute the genetic variability, heritability and genetic advance an investigation was carried out with 575 plants of F 2 population and F 2 derived F 3 progenies from the cross between ICC 13124 and WR315 of chickpea ( Cicer arietinum L.). The genotype ICC 13124 is tolerant to d... (19 Jul., 2013)

Construction of Whole Genome Fosmid Library in Africa Wild Rice (Oryza longistaminata)

Wild rice, Oryza longistaminata , which originated from Africa with the same AA genome as Asian cultivated rice ( O. sativa ), is an important genetic germplasm for the improvement of cultivated rice. In this study, the O. longistaminata fosmid library was constructed that contained 336 000 cl... (19 Nov., 2012)

Developing and Validating the Functional Marker of Rice Waxy Gene, M-Wx

It would be an important significance of developing molecular markers that could effectively track rice amylose content in the breeding program of high-quality rice varieties. In this study the dominant functional marker M-Wx was developed based on the SNP difference that can cause Wx gene func... (25 Oct., 2012)

Design and Validation of Two InDel Markers for Low Glutelin Content (Lgc1) Gene in Rice (Oryza sativa L.)

Rice with low glutelin content is quite effective as a kind of functional food for patients with kidney disease. It is of great importance to develop commercial rice varieties of low glutelin content. In recent years, Lgc1 gene, as an excellent genetic resource for rice varieties with low glute... (07 Sep., 2012)

QTL Mapping and Analysis of Traits Related to Pod Dehiscence in Soybean

Pod dehiscence (Pod shattering) (PD) is an important reproductive trait in soybean, while this trait also causes major yield losses in the mechanically harvested soybeans. In this research, QTL mapping and correlation analysis of three related traits (PD trait (PDH), ratio of thickness to width (... (23 Jul., 2012)

Agrobacterium-mediated Transformation of Cotton Shoot Apex with SNC1 Gene and Resistance to Cotton Fusarium Wilt in T₁ Generation

Using shoot apexes from the upland cotton varieties, named Zhong 35 and Junmian No.1 as explant, the SNC1 , suppressor of npr1-1constitutive cloned from Arabidopsis thaliana , was introduced into cotton genome by Agrobacterium -mediated transformation with the optimized transformation conditio... (09 Jul., 2012)

Construction of Plant Expression Vector with Maize γ-zein Gene and GFP Gene and Their Subcellular Localization

The full length of the maize γ-zein gene amplified by PCR from PROK.TG1LK plasmid was ligated into the intermediate vector pMD18-T, to obtain the recombinant plasmid named pMD18-γ-zein. After the recombinant plasmid and the improved pCAMBIAI1302,respectively were digested by Nco I a... (01 Jun., 2012)

Marker-Assisted Selection of Japonica Restorers used for Yinshui CMS System

In this paper, we employed the rice variety Wanlun 422 with wide compatibility, WA-type restorer line Shuhui 527, Miyang 46, and Miyang 42 as the parent materials, and selected the Japonica rice restorer line for Yinshui type cytoplasmic male sterility (YS-CMS) from the hybrid combinations F 2 ... (02 May, 2012)

Effects of Three Plant Extracts on Growth and Development of Dodder and Soybean and on Protective Enzymes of Host

The ethanol extracts of Melia azedarach bark and leaves, Eucalyptus robusta , leaves, Sapium sebiferum leaves were studied which treated to dodder and its host soybean seedlings in different concentrations, the impact of three plants extracts on dodder and soybean seedlings were evaluated at... (30 Mar., 2012)

Analysis of Characteristics, Mutant Sites and Evolution of Dehydrin 6 (DHN6) Protein in Three Types of Rowed Barleys

Dehydrins (DHNs), a special polypeptide generated in late embryogenesis of higher plants, could protect the plants from the damage caused by cell dehydration. In order to learn the relationship between characteristics and functions of dehydrins, we cloned Dhn 6 genes from three types of rowed ba... (13 Mar., 2012)

Rice Blast Resistance Gene Pi1 Identified by MRG4766 Marker in 173 Yunnan Rice Landraces

Rice Blast is one of the most serious rice diseases and caused great yield losses every year in the world. It had been proved that using resistant rice varieties would be the most effective way to control this disease; therefore, mining the resistant genes might be important foundational work in ... (07 Mar., 2012)

Analysis of Genetic Structure in the USDA Rice Mini-Core Collection Using the SQUAMOSA Promoter-binding-like Gene Family

The SQUAMOSA (SQUA) promoter-binding-like ( SPL ) gene family is a plant specific transcription factor family and has many important biological functions. In this study, 19 pairs of primers were designed based on 19 Oryza sativa L. SQUAMOSA promoter-binding-like ( OsSPL ) gene sequences and use... (20 Jan., 2012)

Mapping and Meta-Analysis of Height QTLs in Soybean

Plant height is one of very complicated quantitative traits of soybean yield and the phenotype data is variable to environment. Since molecular marker technology appeared, many studies about soybean height QTL have been reported. In this study, we analyzed QTLs data of soybean height between 2006... (16 Jan., 2012)

Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (Gossypium) Plant

Cotton ( Gossypium spp ) is an important world crop. Despite the efforts made through traditional breeding methods, cotton breeders still faced with many problems, i.e., narrow genetic base, inability to use alien genes and difficulty in breaking gene linkages. Breeders attempted genetic transfor... (22 Jul., 2011)

Cloning and Expression of an Alcohol Dehydrogenase from Lotus japonicus and Characterization of LjADH1

Alcohol dehydrogenase (ADH), usually using NAD + and NADP + as coenzymes, widely exists in living organisms, It plays extremely important roles in growth, development and stress resistance in plants. In this research, we used diploid Lotus japonicus MG20 (originated from Miyako Island of Japa... (28 Jun., 2011)