Xianju Wang^1,2 , Xuelin Tan¹ , Guojun Shao² , Hui Ma³ , Yajun Chen² , Cheng Zhang² , Wenqi Shang²

1. Rice Research Institute, Yunnan Agricultural University, Kunming, 650201, P.R. China;
2. Rice Research Institute, Liaoning Province, Shenyang, 110101, P.R. China;
3. Shenyang Agricultural University, Shenyang, 110161, P.R. China
Author    Correspondence author
Rice Genomics and Genetics, 2012, Vol. 3, No. 4   doi: 10.5376/rgg.2012.03.0004
Received: 15 Mar., 2012    Accepted: 28 Apr., 2012    Published: 02 May, 2012

This article was first published in Molecular Plant Breeding (Vol.8, No.2, 319-324) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wang et al., 2012, Marker-Assisted Selection of Japonica Restorers used for Yinshui CMS system, Rice Genomics and Genetics, Vol.3, No.4 19-24 (doi: 10.5376/rgg.2012.03.0004)

In this paper, we employed the rice variety Wanlun 422 with wide compatibility, WA-type restorer line Shuhui 527, Miyang 46, and Miyang 42 as the parent materials, and selected the Japonica rice restorer line for Yinshui type cytoplasmic male sterility (YS-CMS) from the hybrid combinations F₂ and F₄ using molecular marker-assisted selection combined with the conventional breeding method. The results showed that the plants containing two restorer genes identified by molecular markers were selected to make hybrid combinations with YS-CMS lines. We found that the seed-setting rate and heterosis of the hybrids were significantly improved. The results of this paper indicate that it is feasible for development of restorers for Japonica YS-CMS line based on molecular marker-assisted.

Yinshui type cytoplasmic male sterility (YS-CMS); Japonica restorer line; Marker-assisted selection; Gamete generation