Lijun Gao^1,2 , Meng Zhou^1,2 , Rentian Chen¹ , Hanliang Gao¹ , Qun Yan¹ , Weiyong Zhou² , Guofu Deng^1,2

1. Guangxi Academy of Agricultural Sciences, Nanning, 530007, P.R. China
2. Guangxi Key Lab for Crop Improvement and Biotechnology, Nanning, 530007, P.R. China
Author    Correspondence author
Rice Genomics and Genetics, 2012, Vol. 3, No. 10   doi: 10.5376/rgg.2012.03.0010
Received: 15 Aug., 2012    Accepted: 19 Oct., 2012    Published: 25 Oct., 2012

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Gao et al., 2012, Developing and Validating the Functional Marker of Rice Waxy Gene, M-Wx, Vol.3, No.10 61-65 (doi: 10.5376/rgg.2012.03.0010)

It would be an important significance of developing molecular markers that could effectively track rice amylose content in the breeding program of high-quality rice varieties. In this study the dominant functional marker M-Wx was developed based on the SNP difference that can cause Wx gene function changes. The effectiveness of the functional marker M-Wx was validated by detecting the rice genotypes of 72 parents and amylose contents. The results showed that 228 bp and 425 bp bands were detected in 43 of 72 parents, genotype assigning to TT, which the amylose content were less than 15.6%, whereas the remaining 29 parents only appeared a 425 bp control band, genotype assigning to GG, which the amylose content were much more than 20.1%. The results of this study indicated the marker M-Wx should accurately detect the level of the amylose content in rice, which and can be applied to improve varieties and enhance rice quality in marker-assisted selection.

Rice; Waxy gene; Amylose; Functional marker