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Isotypes of 78 kD Streptavidin Biotin-binding Proteins (SBP) during Initial Germination of Dark-grown Pea (Pisum sativum L.) Seedlings

In this study two dimensional page and western blotting have been used to investigate the status of isotypes of 78 kD streptavidin biotin-binding proteins (SBP) in dry seeds, germinated seeds and a combination with its different plant parts including stems, plumules and roots of pea ( Pisum sativ... (16 Mar., 2011)

Development of a New japonica Rice Variety Nan-jing 46 with Good Eating Quality by Marker Assisted Selection

In order to developing a new japonica rice variety with both good eating quality and high yield, we employed the high yield japonica rice "Wu-xiang-jing 14" as the maternal parent was crossed to the good quality japonica rice "Kantou 194" with low-amylose content and transluce... (25 Dec., 2010)

Molecular Mapping and Marker Assisted Selection of Soybean Mosaic Virus Resistance Gene RSC12 in Soybean

The P 1 , P 2 , F 1 plants, F 2 population and F 2:3 lines from the cross of Qihuang22×Nannong1138-2 were inoculated with the soybean mosaic virus (SMV) strain SC12 for identification of their resistance in the greenhouse. Qihuang22 and F 1 individuals were resistant(R), and Nannong1138... (08 Dec., 2010)

Colchicine-Induced Trait Variations in Azuki Bean (Vigna angularis)

Seeds of Jinnong 6 variety of azuki bean were soaked in 0.2% and 0.4% concentration of colchicines for 12 hours, respectively. The plant survival rates in M1 generation were 26.7% and 3.5%, and the variation rates were 1.24% and 1.59%, by which it was demonstrated that the higher the concentratio... (20 Oct., 2010)

Rapid and Reliable Purity Identification of F₁ Hybrids of Maize (Zea may L.) Using SSR Markers

Using a rapid and cheap DNA extraction method, genomic DNA extracted from two maize F 1 hybrids and their parental lines was subjected to SSR analysis with 10 pairs of primer. Four of them could detect polymorphism between the male parents and the female parents of the two hybrids, which could b... (30 Nov., 2010)

Characterization and Mapping of Rice Mutants, des2 and des5

Identification of key regulator genes in the control of spikelet development in panicle, one of the main agronomic traits to determine rice yield, is important to investigate the underlying molecular mechanism and provide the molecular basis for analysis of the agronomic traits. In this study, we... (08 Oct., 2010)

Development and Characterization of EST-SSR Markers from NCBI and cDNA Library in Cultivated Peanut (Arachis hypogaea L.)

86 132 ESTs downloaded from GenBank in NCBI and 12 501 ESTs from cDNA library constructed by high-oil linoleic acid accession E12 were analysed. After the preprocession, there were 18 051 singletons and 9 972 contigs in the GenBank of NCBI and cDNA library. Totally 3 104 SSR locis had been screen... (12 Nov., 2010)

Identification of AhAQ1 Encoding a Putative Aquaporin whose Expression is Regulated by Salt Stress In Peanut (Arachis hypogaea L.)

The primary role of aquaporins is to control water transport in plants, which might function in plant response against salt stress. In this research, we obtained the full-length cDNA of an aquaporin gene AhAQ1 by RT-PCR approach from salt-stressed peanut leaves. AhAQ1 encodes a pro... (18 Oct., 2010)

Inheritance Studies of Pisum sativum F₁, F₂ and F₃ Generation based Morphological Traits and Selection of High Yielding Powdery Mildew Resistant Lines

Aim of this s tudy was to determine the inheritance and linkage of six contrasting traits in Pisum sativum F 1 and F 2 generation, and to select high yielding powdery mildew resistant lines in F 3 generation. Two isogenic lines Falloner and 11760-3ER were selected crossed. All the traits in ... (12 Nov., 2010)

Genetic Diversity of Faba Bean Germplasms in Qinghai and Core Germplasm Identified based on AFLP Analysis

In this study we analyzed the genetic diversities of Qinghai’s faba bean ( Vicia faba L.) germplasms based on AFLP techniques. The results showed that the number of effective alleles in landraces, foreign germplasms and released cultivar are 1.065 5±0.126 3, 1.063 4±0.143 0, ... (13 Sep., 2010)

Isolation and Expression Analysis of a Î²-Ketoacyl-Acyl Carrier Protein Synthase â… gene from Arachis hypogaea L.

β-ketoacyl-acyl carrier protein synthase (KAS) plays a pivotal role in de novo fatty acid biosynthesis, joining short carbon units to construct fatty acyl chains by a three-step Claisen condensation reaction in plants and bacteria. A putative KASâ… cDNAs was isolated from peanut by search... (10 Oct., 2010)

Mutations Affecting Lateral Petal Development in Lotus japonicus

To investigate the genetic loci to regulate the development of petal shape, screening of the mutation affecting lateral petal development was conducted in a model plant of legume, Lotus japonicus . Two independent mutants, kew2 and kew3 with the similar phenotype were obtained, whose s... (27 Sep., 2010)

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