Case Study

CRISPR Applications in Rice Breeding: Case Studies of Yield and Stress Tolerance  

Zufang Chen , Deshan Huang , Haiying Huang
Hier Rice Research Center, Hainan Institute of Tropical Agricultural Resources, Sanya, 572025, Hainan, China
Author    Correspondence author
Field Crop, 2025, Vol. 8, No. 1   
Received: 26 Nov., 2024    Accepted: 11 Jan., 2025    Published: 02 Feb., 2025
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

This study explored the application of CRISPR/Cas9 technology in rice breeding, focusing on the analysis of cases for improving yield and stress resistance. CRISPR/Cas9 technology achieves targeted modification of specific genes through precise genome editing, thereby enhancing agronomic traits of rice, such as yield, drought resistance, cold tolerance, and salinity tolerance. Studies have shown that using CRISPR/Cas9 to edit multiple genes simultaneously can effectively improve rice's ability to adapt to environmental stress and increase productivity. In addition, future research should focus on expanding the range of target genes, improving editing efficiency and specificity, and combining CRISPR technology with other breeding methods to accelerate the development of rice varieties with complex traits. CRISPR/Cas9 technology is of great significance in sustainable agriculture, helping to achieve food security and reduce dependence on chemical inputs by developing more resilient and high-yielding rice varieties.

Keywords
CRISPR/Cas9; Rice Breeding; Stress Tolerance; Gene Editing; Yield Improvement
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