Research Report

RAPD Analysis on Genetic Diversity of Cyperus esculentus Germplasm Resources  

Zunmiao Wei1 , Zhanhai Wang1 , Xiangdong Yang2 , Lu Niu2 , Yan Cheng1 , Jiayao Liu1 , Zhongsheng Mu1
1 Economic Plant Research Institute, Jilin Academy of Agricultural Sciences, Changchun, 130033, P.R. China
2 Agricultural Biotechnology Research institute, Jilin Academy of Agricultural Sciences, Changchun, 130033, P.R. China
Author    Correspondence author
Field Crop, 2023, Vol. 6, No. 1   
Received: 18 Feb., 2023    Accepted: 25 Feb., 2023    Published: 03 Mar., 2023
© 2023 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

The research analyzed genetic diversity of nineteen tiger nuts (Cyperus esculentus) and one nutgrass (Cyperus rotundus L.) with RAPD molecular makers. 24 clear and repeatable primers were selected from 96 primers, 94 polymorphic locus were found from 134 locus amplified by selected primers, the ratio of polymorphic locus was 70.15%. Cluster analysis showed that the genetic similarity coefficient was ranged from 0.41~0.46 between tested tiger nut and nut grass; the genetic similarity coefficient among all tiger nuts was range from 0.92~1.00, Tested materials were divided into two groups at the similarity coefficient of 0.40. The result showed that there was high similarity between tested tiger nut materials, they had similar genetic basis, And the seeds from the same region were not gathered into one group; The genetic diversity of tiger nut Germplasm interspecies was rich.RAPD analysis can be used to analyze the genetic diversity of Cypereae Linn Germplasm interspecies.

Keywords
Tiger nut (Cyperus esculentus); Germplasm resources; RAPD analysis; Genetic diversity

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. Zunmiao Wei
. Zhanhai Wang
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. Lu Niu
. Yan Cheng
. Jiayao Liu
. Zhongsheng Mu
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