2 Agricultural Biotechnology Research institute, Jilin Academy of Agricultural Sciences, Changchun, 130033, P.R. China
Author Correspondence author
Field Crop, 2023, Vol. 6, No. 1
Received: 18 Feb., 2023 Accepted: 25 Feb., 2023 Published: 03 Mar., 2023
The research analyzed genetic diversity of nineteen tiger nuts (Cyperus esculentus) and one nutgrass (Cyperus rotundus L.) with RAPD molecular makers. 24 clear and repeatable primers were selected from 96 primers, 94 polymorphic locus were found from 134 locus amplified by selected primers, the ratio of polymorphic locus was 70.15%. Cluster analysis showed that the genetic similarity coefficient was ranged from 0.41~0.46 between tested tiger nut and nut grass; the genetic similarity coefficient among all tiger nuts was range from 0.92~1.00, Tested materials were divided into two groups at the similarity coefficient of 0.40. The result showed that there was high similarity between tested tiger nut materials, they had similar genetic basis, And the seeds from the same region were not gathered into one group; The genetic diversity of tiger nut Germplasm interspecies was rich.RAPD analysis can be used to analyze the genetic diversity of Cypereae Linn Germplasm interspecies.
(The advance publishing of the abstract of this manuscript does not mean final published, the end result whether or not published will depend on the comments of peer reviewers and decision of our editorial board.)
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