Shen Chen¹ , Jie Zhong^1,2 , Xiaoyuan Zhu¹ , Jianyuan Yang¹ , Shengyuan Wu¹ , Liangying Dai² , Liexian Zeng¹

1 Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, P.R. China
2 College of Biosafety Science and Technology, Hunan Agricultural University, Changsha, 410128, P.R. China
Author    Correspondence author
Rice Genomics and Genetics, 2012, Vol. 3, No. 9   doi: 10.5376/rgg.2012.03.0009
Received: 07 May, 2012    Accepted: 31 Aug., 2012    Published: 15 Oct., 2012

This article was first published in Molecular Plant Breeding (2012, Vol.10, No.3, 357-362) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Chen et al., 2012, Genetic Analysis and Gene Detection of Bacterial Blight Resistance in New Released Varieties Lvzhen8072 and Baixiangzhan, Vol.3, No.9 55-60 (doi: 10.5376/rgg.2012.03.0009)

Bacterial blight (Xanthomonas oryzae pv. oryzae, Xoo) is one of the most serious bacterial diseases for rice all over the world. Breeding resistant varieties is one of the most economical and effective measures to control rice bacterial blight. In this research, gene identification and genetic analysis were carried out on the newly released varieties, Lvzhen8072 and Baixiangzhan, in order to provide references in rice production. The cross between Lvzhen8072indica rice cultivar Jingang30 (highly susceptible to Xoo strains) was made to generate F₁ and F₂ population, and then the parents and their F₁ and F₂ individuals were inoculated highly pathogenic GD6027, a V-type strain of bacterial blight in Guangdong Province to identify resistant phenotype. While Xa7-linked microsatellite markers were used to detect the polymorphism of the parents and F₂ individuals in order to confirm the linkage between Xa7 gene and its makers among F₂ individuals. The result confirmed that Lvzhen8072 harbored the resistance gene that isallelic to Xa7, which is independently inherited. In the same way, the bacterial blight resistance gene of Baixiangzhan and its genetic pattern were identified. The result showed that the resistant gene of Baixiangzhan was related to xa5 gene, but the segregation ratio of resistant to susceptible in F₂ individuals didn’t match theoretical segregation radio (1:3) according to the alw of the Mendelian recessive single gene. Obviously, there was segregation distortion obviously existing in the F₂ population.

Rice bacterial blight; Resistance genes; Genetic analysis; Genes detection