Xinguang Cai , Yaodong Liu

Modern Agricultural Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Rice Genomics and Genetics, 2025, Vol. 16, No. 6   
Received: 30 Sep., 2025    Accepted: 15 Oct., 2025    Published: 21 Nov., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Improving the quality traits of rice is an important goal in modern rice breeding, among which the edible taste, appearance quality and nutritional components are all precisely regulated by specific functional genes. Base Editing technology, as a new generation of precision breeding tool, can achieve single-base replacement at specific sites without introducing DNA double-strand breaks, providing an efficient, controllable and safe technical path for the molecular improvement of high-quality rice varieties. This study systematically expounded the experimental process of base editing target design, vector construction and genetic transformation. The functional effect of the editing body was evaluated through molecular detection and quality phenotype determination. The actual case section presented the typical editing results and quality improvement performance of Wx, ALK and OsAAP6. And further discuss the limiting factors of base editing, off-target control and technical optimization directions. This study aims to provide more precise genetic regulatory means for the improvement of rice quality traits, establish an efficient editing system based on the background of superior varieties, lay the foundation for industrial application, and offer a reference path for future targeted editing of "quality design-oriented rice".

Rice quality traits; Base editing; CRISPR/Cas system; Wx gene; Precision breeding