Ziming Ma¹ , Huijiao Bai¹ , Yongmei Jin² , Tao Wu¹ , Rihua Piao³ , Yan Ma¹ , Wenzhu Jiang¹ , Xinglin Du¹

1 College of Plant Science, Jilin University, Changchun, 130062, China
2 Agricultural Biotechnology Institute of Jilin Academy of Agricultural Sciences, Changchun, 130033, China
3 Rice Research Institute of Jilin Academy of Agricultural Sciences, Gongzhuling, 136100, China
Author    Correspondence author
Rice Genomics and Genetics, 2022, Vol. 13, No. 2   doi: 10.5376/rgg.2022.13.0002
Received: 06 Jan., 2022    Accepted: 12 Jan., 2022    Published: 07 Mar., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Ma Z.M., Bai H.J., Jin Y.M., Wu T., Piao R.H., Ma Y., Jiang W.Z., and Du X.L., 2022, Identification, genetic analysis and fine mapping of early senescence mutant es-h in rice, Rice Genomics and Genetics, 13(2): 1-8 (doi: 10.5376/rgg.2022.13.0002)

The early senescence of rice during the reproductive growth period seriously affects rice yield and quality. In this study, ethyl methylsulfonate (EMS) was used to induce the japonica rice variety Hwacheongbyeo to obtain a leaf early senescence mutant during the reproductive growth period of rice, and named as es-h (early senescence-Hwacheongbyeo). Phenotypic analysis showed that the mutant began to have rust spots on the leaves after heading, and withered rapidly with the filling process, the whole plant died off by the fifth week of heading. Agronomic trait analysis showed that, compared with the wild type, the es-h mutant had no significant changes in heading date, panicle length, panicle excertion and panicle number, while significantly reduced in plant height, grain number per panicle, seed setting rate and thousand grain weight. Physiological analysis showed that after heading of es-h mutant, the SPAD value, chlorophyll content, Fv/Fm value and soluble protein content of its flag leaf all decreased sharply. Genetic analysis revealed that the premature aging traits of es-h mutants were controlled by recessive single gene. The target gene was located on the 44.2 kb physical segment of the long arm of chromosome 1 through gene mapping. This study provides a basis for Es-h gene cloning and functional analysis, and molecular mechanism of premature aging.

Rice; Early senescence; e-sh; Fine mapping