Haiya Cai , Shuo Zhang , Gang Liu , Haitao Jia , Aiqing You , Chunhai Jiao

Institute of Food Crops, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan, 430064, P.R. China
Author    Correspondence author
Rice Genomics and Genetics, 2022, Vol. 13, No. 1   doi: 10.5376/rgg.2022.13.0001
Received: 06 Jan., 2022    Accepted: 12 Jan., 2022    Published: 28 Jan., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cai H.Y., Zhang S., Liu G., Jia H.T., You A.Q., and Jiao C.H., 2022, Development and application of identification markers for main parental lines of hybrid rice, Rice Genomics and Genetics, 13(1): 1-8 (doi: 10.5376/rgg.2022.13.0001)

As the development of the modern hybrid rice breeding, many disadvantages behaved in variety identification, variety authenticity identification and seed genetic purity test based on phenotype, which was not suitable for the modern breeding. Insertion-Deletion (ID) loci in rice genome sequence were used to develop 9 ID markers to identify 29 hybrid rice backbone parental lines which were widely used in rice production. Cluster analysis of 29 parental lines were conducted using electrophoresis of those ID markers, and they were clustered into five groups with similarity coefficients of about 0.55. In addition, according to relatedness and original region these lines were clustered into nine subgroups. Genetic purity was conducted for a hybrid rice Guangliangyou476 using ID marker and growth-out test methods, and similar results were generated. So molecular marker could be used into each stage of the modern breeding to accelerate breeding new variety. This research will be helpful for rice breeding, new cultivar registration and seed production.

Hybrid rice; Backbone parental line; Identification marker; Cluster analysis; Seed purity