Danqing Yao

Author    Correspondence author
Rice Genomics and Genetics, 2021, Vol. 12, No.   
Received: 01 Jan., 1970    Accepted: 01 Jan., 1970    Published: 29 Jun., 2021

In this study, the hybrid japonica rice ‘Huayou 14’ and its parents were used as materials, using SSR molecular marker technology, to select primers and identify seed purity according to its polymorphism. From 48 pairs of candidate SSR molecular markers widely distributed on 12 rice chromosomes, 6 pairs of primers were screened, which showed stable codominance between the hybrid and its parents. Completely distinguished, using these primers to carry out molecular purity identification of ‘Huayou 14’ seeds, and comparing the identification results with the field planting purity identification results, the results showed that the identification results of the two were basically the same. Finally, the RM274 and RM1195 marker combinations with the most polymorphic sites and the most significant differences in specificity were finally determined as core primers, and a more rapid and effective technical method for identifying the seed purity of hybrid japonica rice ‘Huayou 14’ was established.