A PCR/LDR-Based Functional Molecular Marker of Rice ( Oryza sativa  L.) Aroma Allele  BADH2-E2

Huangwei Chu<sup>1</sup>; Can Cheng<sup>1</sup>; Rongjian Tu<sup>1</sup>; Jihua Zhou<sup>1</sup>; Fuan Niu<sup>1</sup>; Bin Sun<sup>1</sup>; Yao Li<sup>1,2</sup>; Yao Yao<sup>1,3</sup>; Yiwen Huang<sup>1,3</sup>; Zhongyong Luo<sup>1</sup>; Liming Cao<sup>1</sup>

Research Report

A PCR/LDR-Based Functional Molecular Marker of Rice (Oryza sativa L.) Aroma Allele BADH2-E2

Huangwei Chu¹

, Can Cheng¹

, Rongjian Tu¹

, Jihua Zhou¹

, Fuan Niu¹

, Bin Sun¹

, Yao Li^1,2

, Yao Yao^1,3

, Yiwen Huang^1,3

, Zhongyong Luo¹

, Liming Cao¹

1 Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China
2 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China
3 School of Agricultural Sciences, Jiangxi Agricultural University, Nanchang, 330000, China

Author

Correspondence author
Rice Genomics and Genetics, 2020, Vol. 11, No. 6 doi: 10.5376/rgg.2020.11.0006
Received: 29 Jun., 2020 Accepted: 02 Jul., 2020 Published: 20 Oct., 2020

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Preferred citation for this article:

Chu H.W., Cheng C., Tu R.J., Zhou J.H., Niu F.A., Sun B., Li Y., Yao Y., Huang Y.W., Luo Z.Y., and Cao L.M., 2020, A PCR/LDR-based functional molecular marker of rice (Oryza sativa L.) aroma allele BADH2-E2, Rice Genomics and Genetics, 11(6): 1-6 (doi: 10.5376/rgg.2020.11.0006)

Abstract

The loss-of-function of betaine aldehyde dehydrogenase 2 (BADH2) in rice leading to the accumulation of 2-acetyl-1-pyrrolidine (2AP), the substrate of enzymatic reaction, makes the rice flavor. The rice aroma allele BADH2-E2 has been widely used in aroma rice breeding in China. In this study, a PCR/LDR-based functional molecular marker was developed according to the 7bp deletion in the second exon of badH2-E2 aroma allele. Using PCR/LDR-based markers, the genotypes of Shenfan24, Shenfan26 and their F1 hybrids in the badH2-E2 locus could be accurately identified, and screening the F2 population derived from the cross of Shenfan24 and Shenfan26 shows that the segregation ratio of badH2-E2 positive homozygous, heterozygous and badH2-E2 negative homozygous were 1:2:1. The results of this study provide a new type of molecular marker for marker-assisted breeding and have certain application potential.

Keywords

Rice; Aroma gene; badh2-E2; PCR/LDR; Molecular marker

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Rice Genomics and Genetics

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