Rui Chen , Qingxian Li , Fake Yu , Shaohua Yang , Huaqing Liu , Shufen Zhou , Feng Wang

Fujian Provincial Key Labarotary of Agricltural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, P.R. China
Author    Correspondence author
Rice Genomics and Genetics, 2011, Vol. 2, No. 2   doi: 10.5376/rgg.2011.02.0002
Received: 13 Sep., 2011    Accepted: 20 Oct., 2011    Published: 29 Nov., 2011

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Chen et al., 2011, Genetic and Expression Analyses of a Male Sterile Gene MGA1(t) in Rice (Oryza sativa L.), Fenzi Zhiwu Yuzhong (Molecular Plant Breeding), 9(6): 656-664 (doi: 10.3969/mpb.009.000656)

 

A male gamete sterile mutant (tentatively named Male gametophyte abortion1, mga1(t)) was obtained by abnormality screening of transgenic rice materials in this study. The ratio of marker gene segregation was 1:1 by χ²-test and the pollen grains of the mutant were half sterile. The phenotype of pollen abortion was found co-segregating with T-DNA insertion in mga1(t) mutant, and T-DNA passed only through the female gamete. Southern blot analysis indicated that T-DNA inserted into the genome of the mutant was single copy. Flanking sequence analysis showed that T-DNA was inserted into the 5' untranslated region of a Bax inhibitor (BI)-1 like family protein gene in rice chromosome 3. BI-1 like protein gene was predicted to be involved in programmed cell death. RT-PCR expression analyses revealed that MGA1(t) gene expressed during the whole growth and development stages of rice, and had a strong expression pattern in the spikelets with the length about 0~7 mm. These results indicated that MGA1(t) gene was a male-preferential expressed gene.

Rice; T-NDA; Male gamete; Sterile