

Maize Genomics and Genetics, 2025, Vol. 16, No. 4
Received: 15 May, 2025 Accepted: 30 Jun., 2025 Published: 20 Jul., 2025
Chromatin accessibility plays a key role in regulating gene expression during plant development. While transcriptional changes during maize seed development have been well studied, how chromatin accessibility shifts over time is still not fully understood. This study used ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) to examine open chromatin regions (OCRs) across the maize genome at several seed developmental stages. By combining these data with RNA-seq results, we tracked how accessibility patterns change as seeds grow and found that these changes are closely tied to gene activity. Most OCRs were located near promoters or enhancers. Motif analysis pointed to several transcription factor families—such as bZIP, MYB, and NAC—as likely players in developmental control. Functional annotation showed that OCR-associated genes are highly enriched in pathways like starch biosynthesis, hormone signaling, and embryo development. This study maps how chromatin opens and closes across maize seed development, identifies potential regulatory elements and key TFs, and offers useful insights and targets for epigenetic studies and molecular breeding in maize.
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. Minghua Li

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