Cotton production in Uzbekistan has beendecreasingdue to the increase of impact soil salinization last three-four decades. The use of bacterial fertilizers has been considered as an environmentally friendly methodto improve cotton yield and soil conditions. The research was conductedto evaluate the effect of local produced bacterial fertilizers simultaneously at two sites with moderate saline and non saline soil conditions in Uzbekistan.Our study demonstrated that the seed treatment of cotton plants with plant growth promoting bacterial fertilizers BIST, ErMalxami, Subtinand Fitobisolhad positive impact to the germination, growth and yield of cotton both saline and non saline conditions. The field experiments reveals bacterial fertilizers BIST and Subtin significantly (p<0.05) increased the field seed germination (15%), total shoot and root dry weight (13%~27%), and yield (15%~20%) of cotton in saline soil. On the basis of results, it may be concluded that these beneficial bacterial fertilizers are potential option for improvement of cotton growth and yield in bothsalinatedand not saline soils of Uzbekistan.