Research Report

Identification of QTL Involved in Low-temperature Tolerance at The Germination Stage by Recombination Inbred Lines in Rice  

Suobing Zhang1 , Yunhui Zhang1 , Jing Lin1 , Haiyuan Chen1 , Yingjie Wang1 , Xiaomei Zhu1 , Chunfeng Song2 , Xianwen Fang1
1 The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Institute of Crop Germplasm and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014
2 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014
Author    Correspondence author
Rice Genomics and Genetics, 2020, Vol. 11, No. 3   doi: 10.5376/rgg.2020.11.0003
Received: 05 May, 2020    Accepted: 11 May, 2020    Published: 11 May, 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhang S.B., Zhang Y.H., Lin J., Chen H.Y., Wang Y.J., Zhu X.M., Song C.F., and Fang X.W., 2020, Identification of QTL involved in low-temperature tolerance at the germination stage by recombination inbred lines in rice, Rice Genomics and Genetics, 11(3): 1-6 (doi: 10.5376/rgg.2020.11.0003)


Low-temperature germination (LTG) resistance of direct-seeded rice (DSR) seeds can overcome the developmental delay caused by low-temperature stress and ensure the vigorous growth of seedlings. The LTG is complex quantitative trait controlled by QTLs and has low genetic force, which leads to low efficiency of the direct selection of the trait by breeders. The research on QTL mapping for LTG in rice is helpful to carry out molecular marker-assisted selection (MAS) and improve the efficiency of selection for LTG in DSR breeding. In this study, the LTG rate showed extremely significant differences between the high-quality japonica rice variety Nanjing 46 in Jiangsu and the local variety Zhaxima in Yunnan. The QTL mapping was carried out by a recombination inbred line population (RIL) derived from a cross between Zhaxima and the Nanjing46. Total three QTLs (qLTG-2, qLTG-4 and qLTG-7) involved in LTG were detected on chromosome 2, 4 and 7, respectively. They accounted for 7.69%, 8.75% and 22.93% of the total phenotypic variation (PV), respectively. Interestingly, there was no reported QTL involved in LTG in the candidate region of qLTG-2, which exhibited that the qLTG-2 was a novel QTL locus. The QTLs detected in this study will provide new genetic material and molecular markers for improving the low-temperature tolerance at germination stage in rice by molecular marker assisted selection breeding.

DSR; RIL; LTG; QTL mapping
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. Suobing Zhang
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