Xingzhu Feng

Hainan Institute of Biotechnology, Haikou, 570206, Hainan, China
Author    Correspondence author
Triticeae Genomics and Genetics, 2025, Vol. 16, No. 3   
Received: 30 Apr., 2025    Accepted: 11 Jun., 2025    Published: 29 Sep., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The grain-filling period is a crucial stage that determines the yield and quality formation of wheat grains, involving a complex spatio-temporal regulation process. To gain a deeper understanding of the dynamic changes in gene expression and their molecular mechanisms during grain development, this study, based on multiple time points and multiple tissues, constructed a spatio-temporal transcriptome map of wheat grains covering the entire grain-filling period. Three key periods - early, middle, and late grain-filling - were selected, and the main tissues such as endosperm, embryo, and aleurone layer were collected respectively. High-quality transcriptome data were obtained by using high-throughput RNA sequencing technology. Through differential expression analysis, time series clustering, tissue-specific expression analysis and functional annotation, a large number of key genes and transcription factors related to carbon and nitrogen metabolism, starch synthesis, hormone regulation, stress resistance response, etc. were identified. This study also constructed multiple regulatory networks closely related to grain development and revealed the potential roles of non-coding Rnas and epigenetic factors in regulation, providing resource support for the analysis of the complex regulatory networks of grain development. This research will lay a solid foundation for the improvement of wheat quality, molecular breeding and the functional study of key regulatory genes during the grain-filling period.

Wheat Grouting period; Transcriptome Spatio-temporal map Grain development