BZR (Brassinazole resistant transcription factor) gene family is one specific transcription factor in plant and a critical factor in BRs(Brassinosteroid) transduction pathway, which play a vital regulational role in growth and development, phytohormone signal transduction and responding to abiotic stress. In this study, we conduct the identification of HvBZR gene family in barley by informatics analysis, and accomplished systemic analysis of HvBZR gene family with regard to gene structure, chromosome location, phylogenetic evolution, physicolchemical characteristic, protein conserve motif and transcriptome data analysis and so on. The result of genome wide identification reveals that HvBZR gene family consisted of seven members, which is unevenly distributed in the different chromosomes; the result of gene structure analysis reflects that the length of gene and numbers of introns varied obliviously among the HvBZR gene family members; the protein sequence analysis of physicolchemical characteristic, secondary structure and three dimensional structure reflect that they possessed the similarity; the subcellular location of HvBZRs is distributed in nucleus; the conserved motif analysis of HvBZRs reveals that all HvBZRs contain (pf8657) conserved domain and partial members harboured another domain(pf01373); phylogenetic evolution analysis demonstrated the relationship is closer between the barley and the rice; a lot of cis-element is distributed in the putative promoter region, which is related to growth and development and hormone signal transduction and abiotic stress resoponsiveness based on the analysis of the putative promoter sequence; the expression profiles is distrinct obviously in different tissue and organ during the different development stage based on the transcriptome data. In this research, we conducted the systemic and detailed analysis of HvBZR gene family, in order to providing some theoretical basis for the analysis of the biological function of BZR transcription factors in barley.