Xiaoxi Zhou , Tianxia Guo

Institute of Life Sciences, Jiyang College, Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Legume Genomics and Genetics, 2025, Vol. 16, No. 3   
Received: 03 Apr., 2025    Accepted: 08 May, 2025    Published: 05 Jun., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Adzuki beans (Vigna angularis), as one of the important legume crops in China, are widely used in food, health care and agricultural production. However, they are often threatened by various diseases during their growth process. The NS-LRR (nucleotide-binding site-leucine-rich repeat) gene family is a core gene population in the study of plant disease resistance and plays an important role in pathogen recognition and signal transduction. To systematically analyze the structural characteristics, evolutionary relationships and disease resistance potential of the NBS-LRR gene in adzuki beans, this study conducted the identification and analysis of the NBS-LRR gene family based on whole-genome data. In this study, several NS-LRR genes in the adzuki bean genome were identified and classified into subtypes such as TNL type and CNL type according to domain characteristics. Through phylogenetic tree construction, gene structure analysis and conserved motif comparison, the diversity and evolutionary dynamics of the NB-LRR gene family of adzuki beans were revealed. The expression patterns at different tissues and growth stages were further analyzed. Combined with RNA-Seq and qRT-PCR data, it was found that multiple genes were induced to express in disease-resistant strains and had potential disease-resistant functions. Meanwhile, some key genes also demonstrated correlations with hormone signaling pathways, such as salicylic acid (SA) and jasmonic acid (JA) responses. This research not only enriches the understanding of the disease-resistant genes related to adzuki beans, but also provides a theoretical basis and candidate gene resources for subsequent functional verification and molecular breeding.

Adzuki beans; NBS-LRR; Disease resistance; Evolutionary analysis; Expression spectrum