Research Report

Cloning and Expression Analysis of GmSAP3 Gene in Soybean (Glycine max (L.) Merr)  

Jinyu Zhang , Shaolong Zhu , Maoni Chao , Xinjuan Xu , Pingxi Wang , Zhongwen Huang
Henan Institute of Science and Technology, Henan Collaborative Innovation Center of Modern Biological Breeding, Xinxiang, 453003, P.R. China
Author    Correspondence author
Legume Genomics and Genetics, 2022, Vol. 13, No. 2   doi: 10.5376/lgg.2022.13.0002
Received: 13 Nov., 2022    Accepted: 22 Nov., 2022    Published: 08 Dec., 2022
© 2022 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhang J.Y., Zhu S.L., Chao M.N., Xu X.J., Wang P.X., and Huang ZZ.W., 2022, Cloning and expression analysis of GmSAP3 gene in soybean (Glycine max (L.) Merr), Legume Genomics and Genetics, 13(2): 1-8 (doi: 10.5376/lgg.2022.13.0002)


Stress is an important factor that affecting plant growth and crop yield. Stress Associated Protein (SAP), a type of zinc finger protein, is widely involved in plant development and stress response. In order to explore the function of SAP gene in soybean, we cloned the GmSAP3 gene in Shangdou 1201 and carried out bioinformatics analysis, then the expression pattern of GmSAP3 in soybean tissues and temperature stress was analyzed by real-time PCR. The results showed that the CDS of GmSAP3 was 513 bp and encoded 170 amino acids. The isoelectric point (pI) of GmSAP3 was 6.79 and its molecular weight was 18.30568 kD. GmSAP3 contained two conserved domains (zf-A20 and ZnF- AN1), and evolution analysis showed that GmSAP3 is closely related to GmSAP26. RT-PCR results showed that GmSAP3 mainly expressed in roots and leaves, and GmSAP3 is induced by high temperature stress. It is speculated that GmSAP3 play an important role in soybean stress response. This study may provide theoretical foundation for further research on the function of GmSAP3.

Soybean; Stress associated protein (SAP); Gene clone; Expression analysis
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. Jinyu Zhang
. Shaolong Zhu
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. Xinjuan Xu
. Pingxi Wang
. Zhongwen Huang
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