Cloning and Expression Analysis of GmSAP3 Gene in Soybean (Glycine max (L.) Merr)  

zhangjinyu zhangjinyu
Henan Institute of Science and Technology, Henan Collaborative Innovation Center of Modern Biological Breeding, Xinxiang, 453003, P.R. China
Author    Correspondence author
Legume Genomics and Genetics, 2022, Vol. 13, No. 1   
Received: 21 Nov., 2022    Accepted: 26 Nov., 2022    Published: 22 Dec., 2022
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Abstract

Abstract Stress is an important factor that affecting plant growth and crop yield. Stress Associated Protein (SAP), a type of zinc finger protein, is widely involved in plant development and stress response. In order to explore the function of SAP gene in soybean, we cloned the GmSAP3 gene in Shangdou 1201 and carried out bioinformatics analysis, then the expression pattern of GmSAP3 in soybean tissues and temperature stress was analyzed by real-time PCR. The results showed that the CDS of GmSAP3 was 513 bp and encoded 170 amino acids. The isoelectric point (pI) of GmSAP3 was 6.79 and its molecular weight was 18.30568 kD. GmSAP3 contained two conserved domains (zf-A20 and ZnF- AN1), and evolution analysis showed that GmSAP3 is closely related to GmSAP26. RT-PCR results showed that GmSAP3 mainly expressed in roots and leaves, and GmSAP3 is induced by high temperature stress. It is speculated that GmSAP3 play an important role in soybean stress response. This study may provide theoretical foundation for further research on the function of GmSAP3.

Keywords
Soybean; Stress associated protein (SAP); Gene clone; Expression analysis

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