Characteristics of TaFTSH6 Gene and Expression Analysis under Dry and Heat Stress in Wheat
1 College of Life Science, Linyi University, Linyi, 276000, China
2 College of Life Science and Technology, Xinjiang University, Urumqi, 830046, China
Triticeae Genomics and Genetics, 2021, Vol. 12, No. 1
Received: 05 Jan., 2021 Accepted: 02 Feb., 2021 Published: 17 Feb., 2021
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In order to study the characteristics and functions of wheat FTSH genes, TaFTSH6 was cloned from wheat "bobwhite". Phylogenetic tree analysis showed that the amino acid sequence was very similar to FTSH6 sequence of other species. SMART analysis showed that TaFTSH6 had a conserved N-terminal transmembrane domain, ATP binding sites and hydrophilic Zn2+ metalloproteinase domain. The gene was located on chr7D chromosome of wheat, named as TaFTSH6-7D. Cis-elements analysis of TaFTSH6 promoter sequence showed that there are several drought response sites CAACTG and a heat stress response site GACnnCTCnnGAA in the promoter, as well as many cis-elements related to hormone signal transduction. Quantitative expression analysis of TaFTSH6 gene in wheat seedlings under drought and heat stress showed that both drought and heat stress could increase the expression level of TaFTSH6 gene in root and leaf tissues, which indicated that TaFTSH6 is induced by drought and heat stress. Further study on the function of TaFTSH6 gene in wheat will partly support theoretical guidance to wheat breeding for drought and heat stress tolerance.
Wheat; TaFTSH6; Dry stress; Heat stress
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