Research Report

Homologous Cloning and Bioinformatics Analysis of Gene SWN1 in Miscanthus sinensis  

He Yanceng1 , Yao Li1 , Yuhuan Lin1 , Meng Li1,2 , Zhiyong Chen1,2
1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128
2 Hunan Engineering Laboratory of Miscanthus Ecological Application Technology, 410128
Author    Correspondence author
Rice Genomics and Genetics, 2020, Vol. 11, No. 7   
Received: 08 Aug., 2020    Accepted: 12 Aug., 2020    Published: 12 Aug., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding ( ISSN1672-416X,CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Transcriptional regulation of secondary wall biosynthesis in plants is mediated by a group of secondary wall NAC master switches, In this network, the top-level master switches, secondary wall NACs (SWNs), act together with the second-level MYB master switches to turn on the expression of downstream transcription factors (TFs) and secondary wall biosynthetic genes, which is evolutionarily conserved. To investigate transcriptional regulation of secondary wall biosynthesis in Miscanthus sinensis, the homologous genes ofOsSWN1(SECONDARY WALL NAC DOMAINPROTEIN 1 from Oryza sativa)from Miscanthus sinensis was cloned by a homology-based cloning method in this study, named MsSWN1. Bioinformatics analysis showed that the coding region of MsSWN1 gene is 1 215 bp and encoded 404 amino acids. The relative molecular weight of the encoded protein is 43 181.39D, and its predicted isoelectric point (pI) is 6.57, which is a hydrophilic protein and has a conservative NAC domain. The secondary and tertiary structure prediction analysis showed that α-helix and irregular curl were the main structural elements in the overall protein structure. DNA sequence analysis and phylogenetic tree analysis showed that the protein encoded by MsSWN1 gene is highly conserved in sugarcane, sorghum, and other Gramineae plants, speculating that MsSWN1 gene may also play a regulational function in secondary wall biosynthesis. These results laid the foundation for further digging and verifying the function of MsSWN1 gene, aiming to provide useful information for the future construction of secondary wall biosynthesis regulatory network of Miscanthus sinensis and the efficient biofuel production.
Miscanthus sinensis; SWN1; Secondary wall formation and regulation; Bioinformatics

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Rice Genomics and Genetics
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. He Yanceng
. Yao Li
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. Meng Li
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