Research Report

A PCR/LDR-Based Functional Molecular Marker of Rice ( Oryza Sativa L.) Aroma Allele BADH2-E2  

Huangwei Chu1 , Can Cheng1 , Rongjian Tu1 , Jihua zhou1 , Fuan  Niu1 , Bin Sun1 , Yao Li1,2 , Yao Yao1,3 , Yiwen Huang1,3 , Zhongyong Luo1 , Liming Cao1
Author    Correspondence author
Rice Genomics and Genetics, 2020, Vol. 11, No. 6   
Received: 29 Jun., 2020    Accepted: 02 Jul., 2020    Published: 02 Jul., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding ( ISSN1672-416X,CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The loss-of-function of betaine aldehyde dehydrogenase 2 (BADH2) in rice leading to the accumulation of 2-acetyl-1-pyrrolidine (2AP), the substrate of enzymatic reaction, makes the rice flavor. The rice aroma allele BADH2-E2 has been widely used in aroma rice breeding in China. In this study, a PCR/LDR-based functional molecular marker was developed according to the 7bp deletion in the second exon of badH2-E2 aroma allele. Using PCR/LDR-based markers, the genotypes of Shenfan24, Shenfan26 and their F1 hybrids in the badH2-E2 locus could be accurately identified, and screening the F2 population derived from the cross of Shenfan24 and Shenfan26 shows that the segregation ratio of badH2-E2 positive homozygous, heterozygous and badH2-E2 negative homozygous were 1:2:1. The results of this study provide a new type of molecular marker for marker-assisted breeding and have certain application potential.
Keywords
Rice; Aroma gene; badh2-E2; PCR/LDR; Molecular marker

(The advance publishing of the abstract of this manuscript does not mean final published, the end result whether or not published will depend on the comments of peer reviewers and decision of our editorial board.)
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. Huangwei Chu
. Can Cheng
. Rongjian Tu
. Jihua zhou
. Fuan  Niu
. Bin Sun
. Yao Li
. Yao Yao
. Yiwen Huang
. Zhongyong Luo
. Liming Cao
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