Research Report

Identification of Loss of Rf1 Gene Function Phenomenon in Restorer Line and its Mutant of Dian-Type Hybrid Rice  

Tian Feng1 , Qian Zhu1,2 , Xiaoqiong Guo1 , Wei Li1 , Weilin Yu1 , Shichuan Li1 , Wenbin Wu1 , Ziwei Wan1 , Dajun Huang1 , Lijuan Chen1,2 , Dongsun Lee1,2
1 Rice Research Institute, Yunnan Agricultural University, Kunming, 650201
2 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201
Author    Correspondence author
Rice Genomics and Genetics, 2020, Vol. 11, No. 5   
Received: 15 May, 2020    Accepted: 18 May, 2020    Published: 18 May, 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding ( ISSN1672-416X,CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this study, we carried out a classification survey on sterile plants in the F1 population of high-quality and high-yield Dian-type japonica hybrid rice ‘Dianheyou 34’, combining with the basic agronomic traits and biological information of the backbone restorer line ‘Nan34’ and its mutant Rf1 restorer gene lost mutant, the phenomenon and cause of function loss of restorer lines were preliminarily explored. The results showed that the loss of function with natural mutation at Rf1 in ‘Nan34’ was the main factor that led to sterile plants (two years accounted for 0.26% and 0.79% respectively) in ‘Dianheyou 34’F1 hybrid population; the seed setting rate of ‘Nan34’ wild type was no significant difference compared with its mutant, and the wild type was slightly higher than the mutant in pollen fertility, plant height, ear length and grain length, and slightly greater than the wild type in grain width. By cloning the Rf1 locus of ‘Nan34’ wild type and its mutant, we found that the mutant in ORF region lost a 574 bp long sequence compared with the wild type, which made it unable to encode some PPR proteins. The loss of this sequence may lead to the loss of recovery function. The results lay a theoretical foundation for revealing genetic variation mechanism of restorer genes in three-line hybrid rice breeding and utilization.
Rice (Oryza sativa L); Dian-type CMS; Restorer gene Rf1; ‘Nan34’ mutant, PPR protein

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Rice Genomics and Genetics
• Volume 11
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. Tian Feng
. Qian Zhu
. Xiaoqiong Guo
. Wei Li
. Weilin Yu
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. Wenbin Wu
. Ziwei Wan
. Dajun Huang
. Lijuan Chen
. Dongsun Lee
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