Development of Source Independent Micropropagation System in Dalbergia sissoo Roxb, as a basis for Germplasm Conservation and Disease Free Plants Production
Azeem Iqba Khan1,2
Iqrar Ahmad Rana1
1 Centre of Agricultural Biochemistry and Biotechnology(CABB),University of Agriculture Faisalabad, Pakistan
2 Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
Molecular Plant Breeding, 2016, Vol. 7, No. 15 doi: 10.5376/mpb.2016.07.0015
Received: 10 Dec., 2015 Accepted: 19 Feb., 2016 Published: 19 Feb., 2016
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Preferred citation for this article:
Zaidi S.A., Ijaz S., Iqba A., Khan, and Rana I.A., 2016, Development of Source Independent Micropropagation System in Dalbergia sissoo Roxb, as a Basis for Germplasm Conservation and Disease Free Plants Production, Molecular Plant Breeding,7(10):1-12 (doi: 10.5376/mpb.2016.07.0015)
Plant micropropagation has becoming an imperative strategy at commercial level that provides large number of plants in less time and space. In woody trees, micropropagation is more tedious and cumbersome process than other crop plants. Less success rate in tree micropropagation has been reported in literature. Therefore in this study, expedite, reproducible and source independent micropropagation protocol was developed in Dalbergia sissoo (Shisham) that is an economically important timer yielding tree with medicinal values. For this purpose, eight micropropagation media were studied with various combination of BAP (benzylaminopurine) and IAA (indole acetic acid). Auxially and epicormic shoots of Dalbergia sissoo having at least one nodal bud were used as explants. We studied the impact of these media combination and explant types on micropropagation. We found MPMS5 is the best medium combination that gave maximum number of shoots (2.78 shoots per explant) followed by MPMS7. This selected media combination can be used for Dalbergia sissoo micropropagation as a routine work. Similarly rooting media combinations were also studied for the best root induction in micropropagated shoots of Dalbergia sissoo but little success had been achieved.
Dalbergia sissoo; Micropropagation; Shisham; Tissue culture; Woody tree