Research Report

Bioinformatics and Expression Analysis of GmWRKY32 Gene in Soybean  

Zhao Lin1 , Zhao Jie1 , Sun Yongyuan2 , Cao Jinfeng2 , Liu Jianfeng1
1 College of Life Science, Hebei University, Hebei Baoding, 071002, China
2 Hebei Key Laboratoryof Crop Salt-alkali Stress Tolerance Evaluation and Genetic Improvement, Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou, 061001, China
Author    Correspondence author
Legume Genomics and Genetics, 2021, Vol. 12, No. 1   doi: 10.5376/lgg.2021.12.0001
Received: 18 Feb., 2021    Accepted: 21 Feb., 2021    Published: 28 Feb., 2021
© 2021 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhao L., Zhao J., Sun Y.Y., Cao J.F., and Liu J.F., 2021, Bioinformatics and expression analysis of GmWRKY32 gene in soybean, Legume Genomics and Genetics, 12(1): 1-7 (doi:10.5376/lgg.2021.12.0001)


WRKY transcription factors are very important transcriptional regulators in the process of plant response to plant growth and abiotic stresses. In this study, the open reading frame (ORF) of soybean GmWRKY32 gene was cloned from the WRKY sequence of soybean genome, which was a polypeptide encoding 300 amino acids. Bioinformatics analysis revealed that the WRKY32 protein contained a highly conserved WRKY domain and belonged to Group III of the WRKY gene family. The homology with Glycine soja GsWRKY70, Cajanus cajan CcWRKY70 and Vigna angularis VaWRKY70 proteins was 85%, 79% and 72%, respectively. In addition, GmWRKY32, is an insoluble protein, no signal peptide and transmembrane domain. Further analysis of the gene expression pattern under different stress and hormone induction showed that after 12 h of treatment with PEG, high salt stress and JA, the expression of GmWRKY32 was increased by 4.5, 4.2 and 3.2 times than that of control, respectively. After 24 h of ABA induction, the expression level of GmWRKY32 was increased 6-fold compared with that of the control. Conjecture that it is closely related to the resistance of soybean to abiotic stress which would lay a foundation for the family structure and functional research of soybean WRKY transcription factors, and will provide scientific basis for research of soybean resistance and molecular breeding.

Soybean (Glycine max. L.); GmWRKY32 gene; Bioinformatics analysis; Abiotic stress
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