Genetic Diversity Analysis of Chaenomeles Speciosa (Sweet) Nakai Based on ISSR Molecular Markers
1 Institute of Chinese Medicinal Materials, Hubei Academy of Agricultural Sciences, National Traditional Chinese Medicine Industry Technology System Enshi Comprehensive Test Station, Enshi, 445000
2 Hubei Agricultural Science And Technology Innovation Center Chinese Medicinal Materials Sub-Center, Enshi, 445000
Field Crop, 2020, Vol. 3, No. 6
Received: 04 Jun., 2020 Accepted: 06 Jun., 2020 Published: 06 Jun., 2020
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This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In order to study the genetic diversity and genetic relationship of Chaenomeles speciosa (Sweet) Nakai from different producing areas, 10 ISSR polymorphic primers were used in the polymorphism and cluster analysis of 12 Chaenomeles speciosa (Sweet) Nakai germplasm resources in the main producing areas. The results showed that the percentage of polymorphism of each primer ranged from 65% to 100%, and the genetic similarity coefficient of Chaenomeles speciosa (Sweet) Nakai germplasm resources ranged from 0.505 7 to 0.862 1. The genetic information of various interstitials was abundant. Cluster analysis divided 12 accessions into 3 groups, and the first group was divided into 2 subgroups. Among them, the relationship of M4 (Sangzhi County-1, Hunan Province) and M7 (Sangzhi County-2, Hunan Province) was closest, the relationship of M1 (Eryuan County, Yunnan Province) and M10 (Yunxi County, Hubei Province) was farthest. These results indicate that there is a high genetic diversity of 12 Chaenomeles speciosa (Sweet) Nakai germplasm resources in the main producing areas of Chaenomeles speciosa (Sweet) Nakai in China.
Chaenomeles speciosa (Sweet) Nakai; ISSR molecular marker; genetic diversity; genetic relationship
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